Freshman Adair Landy began her career with the overtime game-winner in Hopkins' 4-3 season-opening win over Mary Washington, and then scored the only Blue Jay goal two days later in a 5-1 loss to Cortland. Sophomore Sarah Bender was another offensive weapon for Hopkins in the early part of the season, scoring all five of her 2005 goals in the first six games. After recording her first point with an assist in the 3-0 win over Washington & Lee, adding two more assists in the 8-3 loss to Salisbury, and tallying a goal and an assist in the 3-2 win over St. Mary's, freshman Leah Horton earned her first start against The College of New Jersey. By then it was clear the rookies would play important roles in the 2005 campaign. JHU opened its conference season with a 3-0 loss to #18 Gettysburg, but quickly rebounded with two straight wins over Muhlenberg, 4-3, and Swarthmore, 3-1. Junior Leah Dudley scored her second game-winning goal of the year against the Mules, while the win over the Garnet evened Hopkins' record at 5-5 and more impressively marked the 100th career win for Callahan in her ninth year with the Blue Jays.

Next came the conference matchup with #12 Franklin & Marshall in which Hopkins exploded for a season-high seven goals. The Blue Jays scored four goals in the first 15 minutes and senior Caite Kappel and junior Jamie Zubrow each finished with two goals, while Landy added one goal and two assists and junior Amy Soergel had a goal and an assist in the 7-0 win. Zubrow, who also converted a penalty stroke in the win over Swarthmore earlier in the week, was named the Centennial Conference Player of the Week as her three goals led Hopkins to two key conference wins.

Meanwhile, Kappel was becoming a major scoring threat as she followed her two-goal performance against F&M with another two goals in the 3-1 win over Dickinson. Three days later, sophomore Kristin Strief scored her first career goal and classmate Lucy Webster - in her first year with the Blue Jays - added another to lead Hopkins to its fifth straight Centennial Conference win. At 8-5 overall and 5-1 in the conference, the Blue Jays were in good position to secure a conference tournament berth.

The five-game winning streak ended with a 2-0 loss to Washington College in the middle of October. The Blue Jays out-shot the Shorewomen 14-6 and earned 20 more penalty corners but could not capitalize in their first loss of the month and first loss at home in 2005. But a 4-0 win over Bryn Mawr improved the Blue Jays to 6-2 in the conference and earned them a spot in the postseason tournament after missing out on a berth the year before. Hopkins ended its regular season with a 4-0 loss to McDaniel and a 3-1 loss to #2 Ursinus to take the fourth seed in the tournament. Johns Hopkins would host fifth-seeded Franklin & Marshall in the first round with the winner advancing to face the top seed and host, Ursinus, in the semifinals. The Blue Jays proved their 7-0 win over F&M in the regular season wasn't a fluke, jumping out to a 3-0 lead on the Diplomats after the first 40 minutes. Horton, Zubrow, and senior midfielder Meighan Roose each scored once in the 3-1 win.

At Ursinus, Johns Hopkins led 1-0 and 2-1 in the first half before the Bears scored three straight to finish with the 4-2 win. Landy set up Kappel for the first goal and then scored a goal of her own off an assist Dudley, but Ursinus was too strong down the stretch. The Bears dominated the Blue Jays 11-1 on shots and 7-2 on corners in the second half. Junior goalkeeper Dayna Eng tied a season-high with 12 saves, as her top four performances came against top-ranked teams. She had 11 saves in the first meeting with Ursinus and against TCNJ, and also recorded 12 at Salisbury. Senior Carly Cammarata provided constant support in front of Eng, starting 18 games and rarely substituting out.

With a 10-9 record, Johns Hopkins fell just short of an ECAC tournament bid and ended the year without a postseason. But the season was a success in all facets; the Blue Jays won six more games than they did in 2004, won six games against teams they lost to the year before, defeated a nationally-ranked team twice, went 6-2 at Homewood Field, and finished fourth in the Centennial Conference. Callahan ended the year with 105 career wins, while Roose finished her career with her fourth straight All-Centennial Conference honor. She was named to the first team for the second time, while Horton was selected to the second team and Landy earned honorable mention status.

Other key contributors to the success of the 2005 season were seniors Shelby Boxenbaum and Kristan Lione, sophomores Megan Maguire, Erin Borek, Lori Vislocky, and Casey Branchini, and freshman Katie Wong, Meghan Marshall, and Emily Miller. Boxenbaum and Lione were solid leaders and substitutes, Vislocky started six games, and Wong improved throughout the season and was a key sub in the final 12 games.